Subcellular fractionation and protease protection.

<p>7.5 ug of mitochondrial protein was processed and loaded in each lane. HB refers to treatment with hypo-osmotic buffer (20 mM KCl) for mitoplast isolation, PK to proteinase K (1 mg/mL) treatment and SDS to sodium dodecyl sulfate (0.5% w/v) treatment for mitoplast lysis. Markers for each compartment include hsp60—matrix, vdac1—outer mitochondrial membrane, tim23—inner mitochondria membrane and cytc—intermembrane space.