Cholesterol efflux to APOAI is inhibited by high glucose.

<p>Bone marrow cells from C57BL/6 mice were differentiated into macrophages under high (25 mM D-glucose) or normal (5.5 mM D-glucose + 19.5 mM L-glucose) glucose. BMDMs were loaded with ³H-cholesterol-Ac-LDL for 24 hours (0.5 μCi/mL ³H-cholesterol and 50 μg/mL Ac-LDL), and then equilibrated in 2 mg/mL BSA media overnight. During equilibration cells were treated with 5 μM T + 1 μM 9cisRA or DMSO vehicle control. Efflux to APOAI (50 μg/mL), or no acceptor (all using 2 mg/mL BSA media) was done for eight hours. Specific efflux to APOAI is represented as a percentage of total efflux over background efflux to no acceptor. Experiment was performed in quadruplicate. Error bars represent SEM. Significance is determined using the two-tailed Student's t-test (*, P < 0.05, **, P<0.01, ***, P<0.001).