Efflux to APOAI is reduced in <i>Prmt2</i>^<i>-/-</i> BMDMs.

<p>Wild type and <i>Prmt2</i>^<i>-/-</i> BMDMs were differentiated under high glucose (25 mM D-glucose) or normal glucose (5.5 mM D-glucose + 19.5 mM L-glucose) and following differentiation were loaded with ³H-cholesterol-Ac-LDL for 24 hours (0.5 μCi/mL ³H-cholesterol and 50 μg/mL Ac-LDL), and then equilibrated in 2 mg/mL BSA media overnight. Efflux to APOAI (50 μg/mL), or no acceptor (all using 2 mg/mL BSA media) was done for 24 hours. Specific efflux to APOAI is represented as a percentage of total efflux over background efflux to no acceptor. Experiment was performed in quadruplicate. Error bars represent SEM. Significance is determined using the two-tailed Student's t-test (*, P < 0.05, **, P<0.01, ***, P<0.001).