Evaluation of Left Ventricle Function by Regional Fractional Area Change (RFAC) in a Mouse Model of Myocardial Infarction Secondary to Valsartan Treatment - Fig 5

<p>(<b>A</b>) Myocyte cross-sectional area (MCSA) in the remote left ventricle (LV) in sham and infarcted groups (n = 6/group). (<b>B</b>) Cell proliferation measured as KI-67 positivity and proliferating fibroblasts detected by double labeling with KI-67 and vimentin. Cells proliferation was expressed as percentage of KI-67 positive cells over total cell number (nuclei), whereas fibroblasts proliferation was expressed as percentage of vimentin/KI-67 double-positive cells over vimentin positive cells. (<b>C</b>) Representative immunofluorescence and quantification of collagen type I staining of the non-infarcted LV (upper panels) and of appendage (lower panels) in sham, MI and MI+Val mice at 4 weeks post-surgery. Interstitial collagen fraction was measured as the percentage of area occupied by collagen on total tissue area. Values are mean ± SEM. * <i>p</i><0.05, *** <i>p</i><0.001 vs. MI. (n = 6/group).