TCRB repertoire analysis for tracking NY-ESO-1-specific CD8⁺ T cell clones.

<p>The frequencies of the 15 most frequent TCRB CDR3 sequences in pretreatment PBMCs (Day 0) and from the NY-ESO-1-specific clones 6-8L and 2F6 were tracked over time during the course of treatment. The known TCRB CDR3 sequences for HLA-B*35-restricted EBV- and HLA-A*24-restricted CMV-specific T cells were also detected in PBMCs. The frequency of each CDR3 sequence is shown. The CDR3 sequence of clone 6-8L was the second most frequent sequence and remained present at high frequency from the initiation of the treatment to three months after the last vaccination. The CDR3 sequence of 2F6 was not detected in PBMCs on day 0; however, it was detected at low frequency on day 106 and day 337.