Effect of metformin on two additional models of breast cancer.

<p>SKBR3 and MDA-MB-231 cells were plated at 8X10⁵ cells/well in 6-well plates in different growth media (MEM 5.5 mM glucose, 25 mM glucose and DMEM) in 6-well plates treated with 10 mM metformin for 24h, and 36h or 48 hours, respectively. After treatment cells were lysed and protein extracts were analysed by Western Blot with anti-mTOR (p) Ser2448, RPS6 (p) Ser240/244 and PARP (Cl) antibodies. GAPDH was used as loading control.