Outline of transfer experiments performed in nude and immunocompetent rats.

<p>Biopsy tissue from GBM patients or passaged xenograft tumors were minced into cubes and allowed to form spheroids in agar-overlay cultures before transplanting to the brain of animals. Comparisons in engraftment were made between 1) immunocompromised nude versus immunocompetent animals implanted with spheroids from the same culture; both primary, low generation and high generation material, 2) primary/low generation versus high generation spheroids in immunocompetent animals, 3) xenografts generated from tumors that engrafted in nude versus immunocompetent animals concerning subsequent engraftment rate in immunocompetent animals.