The N-terminus of FBG1 is required for the degradation, but not binding of A1AT-Z.

<p>Left panel: Input, 4% of total cell lysate co-transfected with A1AT-Z and either pCMV, FBG1, or FBG1ΔN. Right panel: Immunoprecipitation with anti-FLAG coated beads. Separated on SDS-PAGE Stain Free gels, and probed with the indicated antibodies. The lower panel is stained for total protein and shows equal protein loading in all lanes. The middle panel is probed with an anti-FLAG antibody that recognizes both full length and truncated FBG1, demonstrating successful transfection and immunoprecipitation of full length FBG1 and N-terminus deleted FBG1ΔN.