The <i>pkcA</i>^G579R mutant strain is defective in activating the CWI pathway.

<p>Conidia of the wild-type and mutant strains were grown overnight in liquid YG medium and cell wall stress was induced by exposure to CR for 0, 15, 30 and 60 minutes. Samples were collected at the indicated time points for western blot preparation. Phosphorylated and the total fraction of MpkA were detected using anti-phospho p44/42 and anti-p44/42 MAPK antibodies, respectively. γ-tubulin antibody and Coomassie Brilliant Blue stained gel were used as loading sample controls (A). Densitometry analysis of western blots using the ImageJ software presenting the difference in the phosphorylated MpkA/non-phosphorylated MpkA ratio in the wild-type and mutant strain expressed as percentage in the histogram (B).