GFP expression by lenti-GFP-transduced hNSCs. Proliferating lenti-GFP-transduced hNSCs form neurospheres in culture dishes (A) and express GFP (B). Flow cytometry analysis using FlowJo (version 9.3.3) software showed that 94.5 % of all cells are GFP-positive (blue line histogram in C). The red line histogram is the negative control. Scale bar, 100 μm. (TIFF 310 kb
Figure S1. The relative viability of hfNSCs in each group after 2 days of culture under self-renewal...
Brain levels of the β-site of APP cleaving enzyme (BACE-1) mRNA. Expression levels of mRNA were norm...
Figure S5. Modulation of the JAK2-STAT3 pathway does not change expression of proteins involved in A...
hNSCs express diverse trophic factors. (A) In vitro proliferating and differentiated hNSCs expressed...
NSE/APPsw transgenic mouse-derived brain slices treated with BDNF-depleted CM. (A) Western blot show...
The expression of Aβ-degrading enzymes in both in vivo and in vitro. (A) Transplantation of hNSCs (N...
Figure S3. Validation of astrocyte sorting. Normalized expression of cell type specific genes. GFP+ ...
Figure S1. NSCs in culture express p75NTR, sortilin, DCX and SV2. Figure S2. NSCs in culture express...
Sequences of primers used for reverse transcription (RT) and quantitative (q) PCR. (DOCX 25 kb
Figure S1. AAV infect astrocytes selectively. To validate astrocyte tropism of the AAVs used in our ...
is Figure S5 showing mouse NPCs differentiate into mature forebrain cortical neurons. Immunocytofluo...
Analysis of premutation iPSCs and full mutation ESCs. a Immunostaining for pluripotency markers in S...
List of primary antibodies used in immunohistochemistry (IHC) and western blot (WB). (DOCX 28 kb
Figure S2. Sorted GFP+ cells from Nestin floxGFPflox-TK mice give rise to neurospheres. Panel A and ...
FigureS2. Unlike STAT3, STAT1 and Erk are not activated in APP astrocytes. Confocal images of staine...
Figure S1. The relative viability of hfNSCs in each group after 2 days of culture under self-renewal...
Brain levels of the β-site of APP cleaving enzyme (BACE-1) mRNA. Expression levels of mRNA were norm...
Figure S5. Modulation of the JAK2-STAT3 pathway does not change expression of proteins involved in A...
hNSCs express diverse trophic factors. (A) In vitro proliferating and differentiated hNSCs expressed...
NSE/APPsw transgenic mouse-derived brain slices treated with BDNF-depleted CM. (A) Western blot show...
The expression of Aβ-degrading enzymes in both in vivo and in vitro. (A) Transplantation of hNSCs (N...
Figure S3. Validation of astrocyte sorting. Normalized expression of cell type specific genes. GFP+ ...
Figure S1. NSCs in culture express p75NTR, sortilin, DCX and SV2. Figure S2. NSCs in culture express...
Sequences of primers used for reverse transcription (RT) and quantitative (q) PCR. (DOCX 25 kb
Figure S1. AAV infect astrocytes selectively. To validate astrocyte tropism of the AAVs used in our ...
is Figure S5 showing mouse NPCs differentiate into mature forebrain cortical neurons. Immunocytofluo...
Analysis of premutation iPSCs and full mutation ESCs. a Immunostaining for pluripotency markers in S...
List of primary antibodies used in immunohistochemistry (IHC) and western blot (WB). (DOCX 28 kb
Figure S2. Sorted GFP+ cells from Nestin floxGFPflox-TK mice give rise to neurospheres. Panel A and ...
FigureS2. Unlike STAT3, STAT1 and Erk are not activated in APP astrocytes. Confocal images of staine...
Figure S1. The relative viability of hfNSCs in each group after 2 days of culture under self-renewal...
Brain levels of the β-site of APP cleaving enzyme (BACE-1) mRNA. Expression levels of mRNA were norm...
Figure S5. Modulation of the JAK2-STAT3 pathway does not change expression of proteins involved in A...