Differential effects of flavonoids on <i>SREBF2</i> mRNA expression.

<p>The hepatic cells WRL-68 were seeded onto 6-well culture plates and treated with various flavonoids at 1 μM. After 24 h of treatment, total mRNA samples were extracted from the cells. <i>SREBF2</i> mRNA expression was determined using real-time RT-PCR (Fig 1A). Dose-response experiments were performed with luteolin at 0, 0.1, 1, 5, 10 and 25 μM in WRL-68 (upper panel) and HepG2 cells (lower panel) as a follow-up to the screening (Fig 1B). The values are presented as the means ±SEM, n = 3 samples per treatment. Means labeled with (*) are significantly different.