HCV RNA stability in Rab27a-depleted cells.

<p>(A) Effects of Rab27a on HCV RNA decay. Cells were transfected with control or Rab27a siRNAs one day prior to electroporation of HCV RNA. After 3 days post-electroporation, the cells were treated with 25 μM of MK0608, and RNAs were extracted every two hours for 12 hours. HCV RNA abundance was measured by Northern blot. (B) One phase decay graph of HCV RNA. The normalized HCV RNA amounts were determined by Northern blot in three independent experiments. Data were fit to a one-phase decay model (R² = 0.96–0.98). Estimated half-life (t_1/2) of HCV RNA is 4.8 hours in both control- and Rab27a-depleted cells.