Rescue of HCV RNA in Rab27a-depleted cells by ectopic expression of miR-122.

<p>(A) Diagram of experimental design. Huh7 cells were co-transfected with Rab27a siRNA or control siRNA and miR-122 or miR-22 one day before and after virus infection. (B) Overexpression of miR-122 rescues the effect of Rab27a depletion on HCV RNA abundance. HCV RNA abundance during Rab27a depletion and miR-122 overexpression was measured by Northern blot and normalized to actin mRNA. Relative HCV RNA abundance in cells transfected with control siRNA and miR-22 is set to 100%. The data are from four independent replicates (**P<0.005, Student’s t-test).