Time course study of the phosphorylation of several stress-activated kinases after UVB exposure.

<p>(A) Effects on the phosphorylation of ERK/p38/JNK. (B) Effects on the phosphorylation of AKT. Human HaCaT keratinocytes were exposed to UVB at 80 mJ/cm² and were cultured for the indicated times. Whole cell lysates were prepared and were analyzed by Western blot using antibodies to phospho-p38, p38, phospho-ERK1/2, ERK1/2, phospho-JNK1/2 and JNK1/2, and to phospho-AKT and AKT. Representative data from 3 independent experiments are shown.