Decreased 11β-HSD1 activity in the liver and pancreatic islets of MT-IGF mice.

<p><b>A</b>. Changes in DHC conversion rate in pancreatic islets. Radioactivity corresponding to [1,2,6,7-³H] DHC was expressed as percentage of the total radioactivity pooled from all four bands. Also illustrated were the radioactivities of the non-specific band (3. NS) and those remained at the loading spots (4. Origin). <b>B</b>. Changes in DHC conversion rate in the liver. Mean ± SE. N = 3. **P<0.01 vs. wild-type islets or liver. A representative assay was illustrated from three experiments. <b>C</b>. Decreased conversion of human cortisone to cortisol in the islets and liver of MT-IGF mice. Freshly isolated islets and liver microsomes from wild-type and MT-IGF mice were incubated with 500 nM cortisone for 3 h. Steroids were extracted and the concentration of newly converted cortisol was measured using the enzyme immunoassay. N = 3; **P<0.01.