Differentiation of hPSCs on vitronectin variant-coated surfaces into hepatoblasts.

<p>(A and B) H9 human ES cells were induced to differentiate into the hepatic lineage on R-Fc- or NC-Fc-coated surfaces. The cells were fixed and stained at day 5 (definitive endoderm stage) and day 10 (specified hepatic lineages) to detect pluripotency markers (A: POU5F1; B: NANOG) and definitive endoderm markers (A: GATA4; B: SOX17). Inserted figures showed the magnified images. (C) H9 hES and K3 hiPS cells were induced to differentiate into definitive endoderm cells on Matrigel or R-Fc, and the expression of CXCR4 was analyzed by flow cytometry at day 5. Undifferentiated cells were used as a negative control. (D) Differentiated cells were stained for HNF4A (a marker of specified cells) at day 10. Scale bars indicate 100 μm.