Vascular hypoxia in murine breast carcinomas and normal tissue.

<p>Immunofluorescence analysis of hypoxemia in 4T1 mammary gland carcinoma. <b>(A)</b>, 4T1 tumor tissue is stained for tumor vessels (CD31; red). (<b>B)</b> Tumor hypoxia (pimonidazole; green) is co-localized (white) in relation to microvasculature in 4T1 tumor tissue <b>(C)</b>. Quantification of overall tumor vessels <b>(D)</b>, hypoxia <b>(E)</b>, and hypoxic tumor vessels <b>(F)</b> in 4T1, SCK and MMTV-Wnt-1 carcinomas. Immunofluorescence analysis of vasculature (CD31; red) and hypoxia (pimonidazole; green) in non-diseased kidney <b>(G)</b>, spleen <b>(H)</b> and liver <b>(I)</b> indicates a lack of global and vessel hypoxia in normal tissue.