Phenotype of CAP2/<i>Tmprss4</i>-deficient mice.

<p><b>(A)</b> Representative pictures of 3 months old (male) CAP2/<i>Tmprss4</i> wildtype (WT) and CAP2/<i>Tmprss4</i> knockout (KO) littermates. (<b>B</b>) Mean body weight (g) of 3-month-old male and female wildtype (WT, n = 6), heterozygous mutant (HET, n = 11 and n = 9, respectively), and knockout (KO, n = 6 and n = 5, respectively) mice. (<b>C</b>) Relative CAP2/<i>Tmprss4</i> mRNA transcript expression in colon from CAP2/<i>Tmprss4</i>^<i>WT</i>, CAP2/<i>Tmprss4</i>^<i>HET</i> and CAP2/<i>Tmprss4</i>^<i>KO</i> mice (n = 6 mice per group); β-actin is used as internal control. (<b>D</b>) Representative immunoblot showing the presence of a 70kDa CAP2/Tmprss4-specific band in colon extracts from CAP2/<i>Tmprss4</i>^<i>WT</i> (lane 1 and 2), CAP2/<i>Tmprss4</i>^HET (lane 3–5) mice and absence in CAP2/<i>Tmprss4</i>^<i>KO</i> (lane 6–8) mice; arrow indicates the size of the expected but absent CAP2/Tmprss4-specific band in knockouts.