Additional file 8: Figure S6. of Morphological change of CD4+ T cell during contact with DC modulates T-cell activation by accumulation of F-actin in the immunology synapse

The relationship between morphological changes and T-cell activation before and after IS formation. (A) Shape index changes and Ca2+ signals in a CD4+ T cell whose morphology changed to elongated-flattened (left panel). At 1,080 s, IS between CD4+ T cell and DC was formed. Before IS formation, the morphology of CD4+ T cell changed from round to elongated-flattened. At 1,680 s, the morphology of CD4+ T cell changed from elongated-flattened to round-flattened. The peak of Ca2+ signal was occurred before IS formation (at 800 s) and Ca2+ signal sustained at a low level. Images of the morphology and Ca2+ signals of the elongated-flattened T cell before and after IS formations are shown in the right panel. (B) Shape index changes and Ca2+ signals in a CD4+ T cell whose morphology changed to flattened (left panel). Images of the morphology and Ca2+ signals of the round-flattened T cell are shown in the right panel. IS between T cell and DC was formed at 560 s, when Ca2+ signal was at the highest level. The dotted white line depicts the contact boundary between the OT-II CD4+ T cells and the DCs. The calcium intensity was pseudo-colored with hues ranging from blue (low) to red (high). Ca2+ signalling was obtained every 40 s. ICAM-1 was labelled to be green. TCR was labelled to be red. After IS formation, TCR and ICAM-1 were accumulated into the IS of DC-T. Scale bar = 2 μm. (C-E) The distribution of ZAP-70, PLC-γ, PKC-θ (blue) and TCR (red) in the resting CD4+ T cell are shown in panel C to E, reseparately. Scale bar = 2 μm