Quantification of <i>BRAF</i> V600E mutation in cancer cell line DNA using fluorometry.^a

<p>^a For details of the <i>BRAF</i> copy number calculation, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136720#sec005" target="_blank">Materials and Methods</a>. Thus, 50 fM ss DNA corresponds to 3x10⁶ molecules per 100 μL, or 1x10⁶ molecules of genomic DNA in 100 μL of the sample corresponds to the concentration 16.6 fM. Abundance value is determined using ratio of EvaGreen fluorescence obtained for individual analysis on wild-type and mutant specific capture probes as follows: I(<b>CP2m</b>)/(I(<b>CP2m</b>)+I(<b>CP2w</b>)).</p><p>* I (excitation/emission wavelength, nm); background signal is determined by fluorescence measurement of free single-stranded genomic DNA at the same concentration.</p><p>Quantification of <i>BRAF</i> V600E mutation in cancer cell line DNA using fluorometry.<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136720#t002fn001" target="_blank">^a</a>