Lactate dehydrogenase activity in BL cell lines and LCLs.

<p>LDHA catalytic activity was measured in the control cells and after treatment with 100 μM of 10058-F4 for 4 h. The median value of three measurements (standard deviation not exceeding 30%) is shown on the Fig. Each of the four groups consisted of three cell lines: EBV-negative (Ramos, Bl28, DG75), Latency I (BL28/B95.8, Rael, Akata (+)), and Latency III (BL16, BL18, RAJI) BL cell lines, and three LCLs (051128, 111210, and 120214). Kruskal–Wallis tests were applied to the results of eight groups (controls and those treated with 10058-F4). Note the significant decrease of LDHA activity in all the BL lines upon inhibition of MYC transactivation ability (p = 0.0118). The catalytic activity of LDHA was not changed in LCLs (encircled).