Immunofluorescence detection of the acrosome.

<p>(a) Immunofluorescence detection of the acrosome with PNA (green) was performed to illustrate and quantify the sperm acrosome abnormalities. Hoechst was used to nuclei staining (blue); bar = 20μm. (b) The rate of abnormalities including acrosome malformation and deficiency was significantly higher in miR-124 mimic group (41.8%), compared with the control group (24.4%, <i>p</i> = 0.021).