PI3K pathway is active at baseline in 8-chloroadenosine resistant, but not in sensitive, cell lines.

<p><b>a</b>. RPPA heatmap showing proteins that are differentially expressed between the most sensitive and resistant cell lines at baseline prior to treatment. AKT phospho S473 and S308 were higher and phospho AMPK T172, ACC S79, and total PTEN were lower in the resistant cell lines. <b>b</b>. Western blots showing phospho and total AKT and S6 levels in sensitive and resistant cells before and after 40 μM 8-chloroadenosine treatment. Beta-actin served as loading control. <b>c</b>. Western blots showing phospho ERK T202/204 levels in sensitive and resistant cells before and after 40 μM 8-chloroadenosine treatment. Beta-actin served as loading control. <b>d</b>. Quantification of phospho AKT, S6, and ERK and total AKT and S6 levels.