Changes in the EPR spectrum of resting Cygb during the reaction with hydrogen peroxide.

<p>The spectra marked as a, b and c were obtained at 30, 60 and 210 s after the addition of hydrogen peroxide. The inset shows a zoom in the spectra of the free radical produced concomitantly with the formation of high valence species. For EPR experiments, the protein concentration was of 1.2 mmol.L^-1and when present, the peroxide concentration was of 12 mmol.L^-1. These results are representative of three independent replicates.