Changes in the EA spectrum of Cygb during the reaction with <i>t</i>-BuOOH.

<p>A) Bleaching of Soret and Q bands of EA spectra of Cygb in the course of the reaction with <i>t</i>-BuOOH. The black line represents the EA spectrum of resting Cygb, red, green and blue lines corresponds to the spectra obtained at 30, 60 and 200 s after addition of <i>t</i>-BuOOH and indicated by the arrows. B) Normalized spectra of Cygb resting form and 200 s after <i>t</i>-BuOOH addition. C) Differential spectra of Cygb obtained 30 and 200 s after the addition of <i>t</i>-BuOOH. The experiments of EA spectroscopy were performed using 65 μmol.L^-1 Cygb and 0.1 cm optical length. When present, the concentration of peroxides was 650 μmol.L^-1. These results are representative of three independent replicates.