Formation of Cygb amyloid structure after challenge by peroxides.

<p>A), B) and C) show, respectively the epifluorescence images of Cygb control, control plus GSH and challenged by hydrogen peroxide obtained immediately (left panels) 24 h (right panels) after incubation and staining by thioflavine-T. For the low-vacuum SEM experiments, it was used 7 μmol.L^-1 cygb solution with 70 μmol.L^-1 peroxide solutions. For the epifluorescence experiments 70 μmol.L^-1 protein solution was incubated for 1 h with 700 μmol.L^-1 peroxide solution in the presence of thioflavin-T. For FTIR measurements, 7 μmol.L^-1 protein solution was incubated with 70 μmol.L^-1 peroxide solutions for 1 h. The results are representative of three independent experiments.