Identification of the <i>mdtJI</i> promoter region.

<p>The <i>mdtJI</i> transcription start site was located by primer extension of the <i>mdtJI</i> mRNA. The autoradiograph is the result of a typical experiment performed with the <i>mPE2</i> primer on RNA extracted from strain MG1655 pULS85 grown in LB medium at 37°C. Lanes G, A, T, and C show the sequencing ladder generated with the same primer. The sequence of the <i>mdtJI</i> regulatory region is reported in the lower panel. The bold sequence motifs represent the putative -10 and -35 hexamers of the <i>mdtJI</i> promoter and the transcription start site (+1). The MdtJ translation start codon is positioned at +278 (bold) and the coding sequence is underlined. The three putative H-NS binding sites are boxed with a dotted line.