Mucosal IgA from vaginal wash.

<p>ELISA plates were coated with 2 μg/ml of the indicated target protein. Individual vaginal washes were diluted 1:20 in PBS and tested for IgA against (A) VLPs, (B) gp120 Env, and (C) Pr55 Gag. Washes collected before immunization, after intranasal prime, and at time of sacrifice (final) of IgA against (D) VLPs, (E) gp120 Env, and (F) Pr55 Gag. Error bars represent mean ± SEM (n = 8); * <i>p</i><0.05 (2-way ANOVA and Bonferroni Post-Hoc tests versus control and CALV(0)+VLP groups).