Changes in PLB-985 phox Proteins in Pre-Differentiated Cells Exposed to IFN-γ.

<p>A; The western blots are from representative experiments and show the indicated Nox2 proteins in PLB-985 cells that were pre-differentiated for 72 hours in media containing 1.3% DMSO and were then exposed for 3 hours to nothing (lane 1) or 30ng/ml IFN-γ (lane2). Western blotting for GAPDH was done to establish controls for gel loading and transfer efficiency. For gp91phox the protein was detected over a broad range of molecular weights (indicated by the square bracket) due to glycosylation. B; The graphs show the average density of phox proteins measured in 4 experiments like the representative examples in A; the protein densities are expressed as a ratio to the corresponding GAPDH density. For densitometry of gp91phox the lanes were scanned to the extent indicated by the square bracket in the representative gp91phox blot. The error bars are +/- SEM. * indicates a significant difference (p<0.05 by paired t-test).