NanoSight example data of vesicles eluted from the membrane affinity column and the corresponding flow-through.

<p>Vesicles from 4 mL plasma were bound to a membrane affinity column, eluted from the column, and diluted into the working concentration of the NanoSight instrument. The flow-through from the membrane affinity column was kept and processed side by side with the eluate to compare both samples. Shown is the frequency and accumulated fractions of particle size (nm) in both samples types, differing in their average size (peak top noted in picture). While the majority of small protein complexes remains in the flow-through, leading to a small peak size of 99 nm, the eluate from the column contains vesicles, resulting in a larger peak size of 174 nm. The complete data for particle sizes and relative abundance of particles of different fractions from ultracentrifugation and membrane affinity elution is listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136133#pone.0136133.t001" target="_blank">Table 1</a> as mean and standard deviation (SD).