Quantification of RNA yield with increasing plasma volumes.

<p>EV RNA from increasing plasma volumes was extracted with membrane affinity columns and analyzed using RT-qPCR against two mRNAs (BRAF, HPRT1) and a miRNA known to be present in vesicles (let-7a). Shown are raw C_T values with rows as individual extractions and colored diamonds as replicate qPCRs. The signal increase of 1 CT with each doubling of input amount into extraction demonstrates a linear efficiency of EV extraction up to volumes of 4 mL plasma.