NLRP3 expression is up-regulated in human lung tissue during <i>S</i>. <i>pneumoniae</i> infection.
- Diana Fatykhova (789591)
- Anne Rabes (690183)
- Christoph Machnik (789592)
- Kunchur Guruprasad (628625)
- Florence Pache (789593)
- Johanna Berg (789594)
- Mario Toennies (789595)
- Torsten T. Bauer (598944)
- Paul Schneider (755525)
- Maria Schimek (789596)
- Stephan Eggeling (789597)
- Timothy J. Mitchell (150478)
- Andrea M. Mitchell (150475)
- Rolf Hilker (789598)
- Torsten Hain (57669)
- Norbert Suttorp (167418)
- Stefan Hippenstiel (167416)
- Andreas C. Hocke (789599)
- Bastian Opitz (334645)
Publication date
August 2015
DOIAbstract
<p>(A, B) Human lung tissue was infected with 1x10<sup>6</sup> CFU/mL <i>S</i>. <i>pneumoniae</i> serotype 2 D39 or D39Δ<i>ply</i> and expression of NLRP3 was assessed by immunoblotting. (C) NLRP3 inhibitor glybenclamide efficiently reduced IL-1β secretion in human lung tissue during <i>S</i>. <i>pneumoniae</i> infection. Human lung tissue was preincubated with the inhibitor for 2 h and infected with 1x10<sup>6</sup> CFU/mL <i>S</i>. <i>pneumoniae</i> D39 for 16 h. IL-1β production was quantified by ELISA. Data are shown as mean ± SEM of three (A, B) or six (C) independent experiments carried out in duplicates. Significance is indicated by asterisks *** = p<0.001.</p
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