Characterization of SC-ASC and V-ASC from obese and lean mice.

<p><b>A</b>, Morphology of adherent cells showing similarity of SC-ASC and V-ASC from obese and lean mice. Scale bar, 1μm. <b>B</b>, Adipogenesis of SC-ASC and V-ASC. Differentiated cells were stained with oil red-O and quantified by percentage of pixels of oil red-O in images. Error bars, SEM. **, <i>P</i> < 0.01(Mann-Whitney test). Scale bar, 100μm.<b>C</b>, Osteogenesis of SC-ASC and V-ASC. Differentiated cells were stained with alizarin red S. Error bars, SEM. **, <i>P</i> < 0.01(Mann-Whitney test). Scale bar, 100μm. <b>D</b>, Quantitative RT-PCR analysis of mRNA levels for genes hallmarking adipocyte differentiation and lypolysis normalized to 18s RNA expression in indicated ASC at baseline and 14 days after adipogenesis induction. Error bar: SEM.