<p>Mice were immunized twice at weeks 0 and 3 with Vrep or conventional DNA vaccines as described in Materials and Methods and shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136635#pone.0136635.t001" target="_blank">Table 1</a>. The different immunization groups are denoted by the initial vaccine given at week 0 followed by the second vaccine given at week 3 (eg. DNA-control:DNA-Acr/Ag85B). Splenocytes were harvested at 3 wk post-immunization and both Acr and Ag85B-specific CD4+ (A) and CD8+ (B) T cell responses were assessed by IFN-γ ELISpot assay. Data shown are mean counts of spot forming cells (SFCs) ± SEM from pooled splenocyte samples (n = 5) in a single experiment and are representative of three such ...
<p>(A) AG129 mice were immunized with 1×10<sup>6</sup> IU DENV2 E85-VRP i.p. 42 and 33 days before c...
<p>Groups of mice (n = 6/group) were primed i.d. with Ad5, MVA or Ad5+M expressing Ag85A and receive...
(A) Splenocytes from mice immunized with the DNA vaccine candidates were collected 1 week after the ...
<p>Mice were immunized twice at weeks 0 and 3 with Vrep-Luc or Vrep-Acr/Ag85B vaccines as described ...
<p>Mice were immunized twice at weeks 0 and 3 with Vrep-Luc or Vrep-Acr/Ag85B vaccines as described ...
<p>Mice were immunized twice at 3 or 6-week intervals with Vrep vaccines and splenocytes and lung ly...
<p>Mice were immunized as described in <a href="http://www.plosone.org/article/info:doi/10.1371/jour...
<p>Mice were immunized twice via the IM route with DNA vaccine encoding Ag85B and/or flagellin as sh...
<p><b>A</b> & <b>B</b>) Balb/c mice were immunized i.m. at weeks 0 and 2 with 50 µg of pSG2-Ag85A or...
<p>C57BL/6 mice were immunized with an empty vector, cytokines only, or D/P vaccine (V1 dose: TcG2- ...
<p>C57BL/6 mice were vaccinated with 25 μg of the indicated plasmids on days 0 and 21. One day follo...
<p>Δ <b>boosts.</b> (A) Schematic schedules of DNA prime/m8Δ-Env boost vaccination protocol. Mice we...
<p>One week after the second and final immunization splenocytes were analyzed for intracellular cyto...
<p>(A) Mice (<i>n</i> = 3 per group) were immunized once with 5 μg of DREP-C-ENV or 5 μg of DREP-C-G...
<p>CB6F1 mice were vaccinated once with Ad-H4, twice with rH4 in cationic liposomes, or once with rH...
<p>(A) AG129 mice were immunized with 1×10<sup>6</sup> IU DENV2 E85-VRP i.p. 42 and 33 days before c...
<p>Groups of mice (n = 6/group) were primed i.d. with Ad5, MVA or Ad5+M expressing Ag85A and receive...
(A) Splenocytes from mice immunized with the DNA vaccine candidates were collected 1 week after the ...
<p>Mice were immunized twice at weeks 0 and 3 with Vrep-Luc or Vrep-Acr/Ag85B vaccines as described ...
<p>Mice were immunized twice at weeks 0 and 3 with Vrep-Luc or Vrep-Acr/Ag85B vaccines as described ...
<p>Mice were immunized twice at 3 or 6-week intervals with Vrep vaccines and splenocytes and lung ly...
<p>Mice were immunized as described in <a href="http://www.plosone.org/article/info:doi/10.1371/jour...
<p>Mice were immunized twice via the IM route with DNA vaccine encoding Ag85B and/or flagellin as sh...
<p><b>A</b> & <b>B</b>) Balb/c mice were immunized i.m. at weeks 0 and 2 with 50 µg of pSG2-Ag85A or...
<p>C57BL/6 mice were immunized with an empty vector, cytokines only, or D/P vaccine (V1 dose: TcG2- ...
<p>C57BL/6 mice were vaccinated with 25 μg of the indicated plasmids on days 0 and 21. One day follo...
<p>Δ <b>boosts.</b> (A) Schematic schedules of DNA prime/m8Δ-Env boost vaccination protocol. Mice we...
<p>One week after the second and final immunization splenocytes were analyzed for intracellular cyto...
<p>(A) Mice (<i>n</i> = 3 per group) were immunized once with 5 μg of DREP-C-ENV or 5 μg of DREP-C-G...
<p>CB6F1 mice were vaccinated once with Ad-H4, twice with rH4 in cationic liposomes, or once with rH...
<p>(A) AG129 mice were immunized with 1×10<sup>6</sup> IU DENV2 E85-VRP i.p. 42 and 33 days before c...
<p>Groups of mice (n = 6/group) were primed i.d. with Ad5, MVA or Ad5+M expressing Ag85A and receive...
(A) Splenocytes from mice immunized with the DNA vaccine candidates were collected 1 week after the ...