Spatiotemporal expression pattern of <i>PaFD</i> and its functional activity in <i>Arabidopsis</i>.

<p>A, Developmental stages of floral buds and spikes are described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134987#pone.0134987.g001" target="_blank">Fig 1</a>. B, <i>PaFD</i> expression in a developing spike by <i>in situ</i> hybridization. The magnified axillary floral meristem in the emerging spike (≤ 3 cm in length) is in the box (left panel) and the middle panel is a negative control with a sense probe. <i>PaFD</i> transcript was detected in the floral meristems in the developing spike (3–10 cm in length, right panel). Bar is 100 ìm. C, Expression pattern of <i>PaFD</i> in different developmental stages. The stage 1 (S1) is 16-month and the S2 is 20-month old stages of the orchid in the flasks. S3 and S4 show 26-month and 34-month old stages of the orchid in the pots, respectively. Bar is 1 cm. D, Flowering phenotypes of wild type, <i>fd</i> mutant (<i>fd-3</i>), and two independent homozygous transgenic plants expressing <i>PaFD</i> under the control of <i>Arabidopsis FD</i> promoter in <i>fd-3</i> background. E, Flowering time of the each genotype shown in D. Twelve individuals for each genotype were used for flowering time measurement and <i>PaFD</i> expression is detectable only in the transgenic plants.