Phenotypic analysis of the <i>ZmIRT1</i>-overexpressing <i>Arabidopsis</i> (OX9, OX10, OX56) and wild-type (WT) plants under various metal conditions.

<p>(A) Schematic diagram of pBI121-<i>ZmIRT1</i> construct. (B) RT-PCR verifying the expression of <i>ZmIRT1</i> in transgenic lines. <i>AtUBP6</i> was used as an internal control. The growth trend of 2-week-old seedlings under (C) standard nutrient conditions (MS medium), (D) Zn, and (F) Fe-deficiency (Zn- and Fe-), as well as (E) 200 μM ZnSO₄ (Zn++) and (G) 300 μM FeSO₄ (Fe++) treatment. (H) The root lengths of the wild-type and transgenic plants were measured using the ImageJ software. Error bars represent standard errors. Asterisks denote significant differences: *P<0.05, **P<0.01.