Polyglutamine-expanded Huntingtin exon 1 forms aggregates in yeast that can be isolated by TAPI.

<p>(A) Fluorescence microscopy of yeast expressing GFP-tagged Huntingtin exon 1 (Htt) with a normal (Q25) or an expanded polyglutamine tract (Q103). (B) Western blot of GFP-Htt-Q25 and GFP-Htt-Q103 showing that high molecular weight aggregates can be isolated from Htt-Q103 expressing yeast cells. Lysate = input; TAPI = purified aggregate.