Reduced CD8⁺ and effector CD8⁺ T cells are observed in absence of M1 expression.

<p>8–12 week old IFNγR-/- C57Bl/6 mice were intranasally infected with 1x10⁵ pfu MHV68 (WT or M1st) and sacrificed at 28 days post infection. Whole lungs were harvested and assessed for presence of CD8⁺ T cell populations and effector function (n = 5 mice/group). Mean and std. error are shown for (A) absolute number of lung and CD8⁺ T cells, (B&C) absolute number of tetramer specific and peptide responsive CD8⁺ T cells, with MFI of cytokine expression, (D) absolute number of Vβ4⁺ CD8⁺ T cells and M1 responsive CD8⁺ T cells. Statistics were measured using a Mann-Whitney 2 tailed test (* <i>P</i> = 0.0119, ** <i>P</i> = 0.0079, * <i>P</i> = <0.0004).