Time-dependent activation of caspases in ethanol-exposed HL-1 cells.

<p>(A) Activation of caspases in HL-1 cells occurs after 24–48 hours of exposure to 2% ethanol. Cells were incubated in medium containing 2% ethanol for the indicated time periods, and the levels of the cleaved (activated) forms of caspase-9 and -3 were determined by western blot analysis. Hsc70 served as a loading control. Quantitative analysis of cleaved caspase-9 (B) and -3 (C) is shown. Relative levels to ethanol (48 h)-treated group were shown. Each graph shows mean±S.E. of 6 samples. **, p<0.01.