Detection sensitivity of the PCR-GO system.

<p>Left, The RFU ratios (sample/control) increased in proportion to the concentration of p-IS<i>6110</i> used for PCR amplification (10¹–10⁶ copies/reaction). Negative group (no template control) was significantly different from positive groups (10¹, 10², 10³, 10⁴, 10⁵, 10⁶ copies/reaction) (Tukey-Kramer multiple-comparison test, <i>p</i> < 0.05). 10 replicates were performed at each concentration. Right, Gel electrophoresis of the 113-bp amplicons after PCR amplification using 10¹–10⁶ copies of the p-IS<i>6110</i> plasmid per reaction, as indicated. Right, C, blank; NC, no-template control.