Stable global DNA methylation and hydroxymethylation and HMG-CoA reductase DNA hypermethylation in diet-induced NAFLD.

<p>(A-B) Although the gene expression for <i>Dnmt1</i> and <i>Dnmt3a</i> tended to decrease and decreased, respectively, the percent methyldeoxycytidine did not change between chow and HFHC group. (C) The relative 5hmdC concentrations also did not change. (D) Among the individual genes involved in the pathogenesis of NAFLD, <i>Hmgcr</i> was hypermethylated (p< 0.01) but there were no methylation changes for <i>Fasn</i>, <i>Nfκb1</i>, <i>c-Jun</i>, <i>Bcl-2</i>, and <i>Caspase 3</i>in HFHC group. Data are represented as mean ± SEM. Legend: B-cell lymphoma 2 (Bcl-2); DNA methyltransferase 1 and 3a (Dnmt 1 and Dnmt3a); fatty acid synthase (Fasn); 5-hydroxymethyl-2’-deoxycytidine (5hmdC); 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase, Hmgcr); nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (Nfkb1).