Clinical and molecular features of the 3 patients investigated by high-throughput sequencing.

<p>Three patients, 2 showing a pattern compatible with a direct evolution and 1 consistent with the existence of a CPC, all having a IgH-VH3 family clonal rearrangement were included in the study.</p><p>Samples AD1548 and 1787 from pt1, shown in italic, were not included in the high-throughput sequencing analysis of IgH-VH but only in the SNP CNV study.</p><p>^±The sample ID corresponds to the cell suspension vial ID or DNA identification number.</p><p>P = progression, R = relapse, SD = stable disease; subsequent number relate to episode e.g. R2 = second relapse</p><p>N.A. = not available SHM = somatic hypermutation</p><p>FL = Follicular Lymphoma; t-FL = transformed Follicular Lymphoma</p><p>MBR stands for Major Breakpoint Region and mcr for minor cluster region</p><p>* Pt2 has 2 different mutations at the same base (a pattern compatible with direct evolution)</p><p>^# Samples R8403FL1 and R9129FL2 had 2 different DH/JH rearrangements but when aligned they showed the a similar CDR3 region, with additional 21 insertions in the sample R8403FL1 (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134833#pone.0134833.s006" target="_blank">S6 Fig</a>)</p><p>Clinical and molecular features of the 3 patients investigated by high-throughput sequencing.