Adoptive transfer of normal Treg cells into BA mice suppresses RRV-induced generation of Th17 cells.

<p>Saline or RRV was injected within 12 hrs of birth. Tregs were injected i.p. 3 days post infection. <b>(A)</b> Representative FCM diagrams of Th17 cells from liver of RRV primed 7 days old mice. *** <i>P</i><0.001, ** <i>P</i><0.01, n = 11. <b>(B)</b> Hepatic mRNA for genes encoding IL-17A and its transcription factor ROR-γt were quantified by real-time PCR and expressed as fold change (vertical axis) in mice with BA over controls, n = 9 for biliary atresia and n = 7 for controls; all fold changes were statistically significant at * <i>P</i><0.05, ** <i>P</i><0.01. <b>(C)</b> Immunofluorescence staining of liver frozen sections of mice at day 7 post infection. Antibodies to CD4 (Green) and IL-17A (Red) were added to distinguish CD4+ cells and IL-17A+ cells. Antibodies to Cytokine 7 (Khaki) indicate the bile duct lumen. Nucleus were stained by DAPI (Blue). Magnification x200. * <i>P</i><0.05. Representative of 4 experiments.