The proportion of Treg decreases and the proportion of Th17 cells increases, but the absolute numbers of both are increased.

<p><b>(A and B)</b> At the time of bile ductal obstruction, flow cytometry analysis showed that the percentage of Tregs decreased but Th17 increased in the liver of the RRV group compared to the saline group. The percentage of CD4⁺ cells positive for the indicated marker is shown. <i>P</i> = 0.0245 for the Treg group and <i>P</i><0.001 for the Th17 group, n = 11 for each group. <b>(C)</b> Absolute numbers of Tregs increased 2.6 fold and Th17 increased 34 fold in the RRV challenged group compared to the saline control group. Numbers were measured per individual liver by FCM, * <i>P</i><0.05, ** <i>P</i><0.01, n = 5 for each group.