<i>GATA-2</i> 1S promoter activity is associated with the +9.9 kb enhancer.

<p>(A) The enhancer activities of the constructs, which included <i>GATA-2</i> upstream or intron regions with various combinations of <i>GATA</i> binding sites, CCAAT/enhancer binding protein, alpha (<i>CEBPA</i>) binding sites, and an E-box. YN-1 cells were transiently cotransfected with Renilla (pRL) and luciferase vectors (Luc). The luciferase vector was either empty or contained the <i>GATA-2 1S</i> promoter cloned upstream of luciferase (1SLuc), with or without the upstream 1S promoter sequence and/or +9.9 kb site. Luciferase activity was measured 24 h after the first transfection. Results are presented as ratios of luciferase activities (means of Firefly/Renilla ± standard error, n = 3). (B) Impact of the GATA-binding site and/or E-box site mutation on the +9.9/1S promoter activity. We generated wild-type, E-box only, GATA-binding site only and double-mutation constructs fused to a luciferase reporter gene and performed a transient transfection assay in YN-1 cells (mean ± standard deviation, n = 3). * p<0.05.