Distinct pattern of miRNA levels in sciatic nerve in response to injury.

<p>The heatmap with a cluster dendrogram showed significant changes in levels of 63 miRNAs in rat sciatic nerve that received a crush injury and were sacrificed at 1, 4, 7, and 14 days post-injury (DPI), as compared to the sham-operated uninjured control (n = 3/group). <b>A.</b> The color scale shown on the top left denotes the relative expression level of the indicated miRNA across all time points (log₂ scale): red represents an increased change in level and green denotes a decreased level. Clustering analysis was performed using <i>Cluster 3</i>.<i>0</i> with an average linkage and Euclidean distances metric and visualized using Java <i>TreeView</i>. <b>B.</b> Each column represents different time points after injury [1, 4, 7, and 14 days post-injury (DPI)], as compared to the uninjured control (n = 3/group), and rows represents individual miRNAs. Red represents an increased change in level compared to that in the uninjured control and green denotes a decreased level. Insets on the top show three distinct clusters of miRNA changes. Dashed lines indicate miRNA levels in the uninjured control.