IF analyses using anti-protein VII antibodies.

<p>(A) IF analyses with mouse and rat anti-protein VII antibodies. U2OS cells were either mock-infected or infected with Ad5 and at 3 hpi subjected to IF analyses with mouse (green) and rat anti-protein VII antibodies (red). Merged images with DAPI staining (gray) are also shown. (B) IF analyses with rabbit and rat anti-protein VII antibodies. IF analyses were carried out as described in (A) but using rabbit (cyan) and rat anti-protein VII antibodies (red). Arrows indicate cytoplasmic protein VII signals. (C) IF analyses with Alexa-labeled viruses. U2OS cells were first incubated with Alexa488-labeled Ad5-GFP (green) at 4°C for adsorption, and then transferred to 37°C. After 0, 20, and 60 min, cells were collected for IF analyses using rabbit (cyan) and rat anti-protein VII antibodies (red). Asterisks indicate protein VII signals at the nuclear rim. (D) IF analyses using cells at late phases of infection. U2OS cells were either mock-infected or infected with Ad5, and at 24 hpi subjected to IF analyses using either mouse and rat (green and red, left panels) or rabbit and rat anti-protein VII antibodies (cyan and red, right panels). DAPI staining (gray) and merged images were shown for mock and infected cells, respectively. For right panels, magnified images of the regions marked by squares are also shown.