Model simulation of metabolic fluxes.

<p>Metabolic fluxes (all in mmol.10^-6cells.h^-1) were simulated for the same conditions as in Figs <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136815#pone.0136815.g002" target="_blank">2</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136815#pone.0136815.g004" target="_blank">4</a> for batch cultures using Biogro-CHO (straight line—black) and PowerCHO-2 (dashed line—green), and fed-batch cultures using Biogro-CHO (dotted line—red) and PowerCHO-2 (dashed and dotted lines—blue). Ammonia production (Fig 7F) is defined as <i>V</i>_<i>GLDH</i>+<i>V</i>_<i>GlnT</i>+<i>V</i>_<i>SDHH</i>+<i>V</i>_<i>ASN</i>+<i>V</i>_{<i>HISARGTA</i>,} and TCA cycle flux (Fig 7G) is defined as the sum of the reactions entering in (<i>V</i>_<i>GLDH</i>+<i>V</i>_<i>PDH</i>+<i>V</i>_<i>AlaTA</i>+<i>V</i>_<i>PC</i>+5*<i>V</i>_{<i>AAtoSUC</i>}), and finally the net ATP production flux (Fig 7I) consists in the sum of the ATP-producing metabolic fluxes (<i>V</i>_<i>PGK</i> + <i>V</i>_<i>PK</i> + <i>V</i>_<i>AKGDH</i> + <i>V</i>_<i>PC</i> + <i>V</i>_<i>GlnT</i> + 2*2.5*<i>V</i>_<i>resp</i>).