Additional file 10: of Genome-wide replication landscape of Candida glabrata

Effect of smoothing on replication curve shape. The chromosome G replication curve is represented after smoothing with different span values, from 0.0001 (almost no smoothing) to 0.075 (extensive smoothing). With low levels of smoothing, several peaks or shoulders did not correspond to ARS positions and were clearly artifacts (0.015 span value). This effect was particularly pronounced for small chromosomes. With extensive smoothing (0.075 span value), close replication peaks tended to merge into one single peak, like, for example, the large left-most double (or triple) replication peak. Comparison of different smoothing levels with ARS positions (red dots) led us to use a 0.04 span value for all chromosomes. (PDF 1101 kb