Additional file 2: Figure S2. of Progenitor potential of nkx6.1-expressing cells throughout zebrafish life and during beta cell regeneration
- AurĂŠlie Ghaye (3562196)
- David Bergemann (3562187)
- Estefania TarifeĂąo-Saldivia (3562199)
- Lydie Flasse (3562190)
- Virginie Von Berg (173736)
- Bernard Peers (61486)
- Marianne Voz (3562193)
- Isabelle Manfroid (61481)
Publication date
September 2015
DOIAbstract
The bacterial artificial chromosome (BAC) reporter line Tg (ascl1b:eGFP-creER T2 ) mirrors the expression of the endogenous ascl1b gene. A Schematic representation of the â61 to +89 kb ascl1b:eGFP-2A-creER T2 BAC transgene. By BAC recombineering using galK selection, the eGFP-2A-creER T2 cassette is introduced into exon 1, replacing the beginning of the ascl1b open reading frame (aa 1 to 163). B Epifluorescence microscopy images of the immunodetection of GFP in 17-hpf Tg(ascl1b:eGFP-creER T2 ) embryos. C, D Visible WISH showing expression of endogenous ascl1b in a wild-type (WT) embryo (C) and of GFP in Tg(ascl1b:eGFP-creER T2 ) embryos (D) at 15 hpf. Lateral views with the anterior part to the left. P pancreas. (PNG 1068 kb
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